Shona Mookerjee, PhD

Biography

Since joining the College of Pharmacy in 2013, I have taught across a wide range of topic areas in the biological and pharmaceutical sciences, with a particular emphasis on pharmacology. My research program, housed at the Buck Institute for Research on Aging, focuses on bioenergetics and mitochondrial function, with applications to cancer metabolism, drug screening, and cellular physiology.

Education

• B.A., Biology and Environmental Sciences, Carleton College
• Ph.D., Molecular Genetics, University of Rochester

Recent Publications

2021    Chun, S.C., Yousif, F., Doroudgar, S., Mookerjee, S.A.. Identifying Determinative Matching Factors in a Pharmacy Faculty Mentoring Program. American Journal of Pharmacy Education, submitted for publication, October 2021.

2021    Mookerjee, S.A., Gerencser, A.A., Watson, M.A, Brand, M.D. Controlled power: how biology manages succinate-driven energy release. Biochemical Society Transactions, submitted for publication Aug 2021.

2021    Labbé, K., Mookerjee, S.A., Gibbs, E., Lerner, C.A., Nunnari, J. The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis. Journal of Cell Biology 220: e202103122, jcb.202103122

2020    Louie, M.C., Ton, J., Brady, M.L., Le., D.T., Mar, J.M., Lerner, C.A., Gerencser, A.A., Mookerjee, S.A. Total Cellular ATP Production Changes With Primary Substrate in MCF7 Breast Cancer Cells. Frontiers in Oncology 10:1703 doi:10.3389/fonc.2020.01703

2019    Handel, M.E., Brand, M.D., Mookerjee, S.A., The Whys and Hows of Calculating Total Cellular ATP Production Rate. Trends in Endocrinology and Metabolism 30: 412-416 doi: 10.1016/j.tem.2019.04.007.

2018    Mookerjee, S.A., Quinlan, C. L., Wong, H., Dighe, P, Brand, M.D. Plate-based measurements of respiration by isolated mitochondria Methods in Molecular Biology 1782: 301-313. doi 10.1007/978-1-4939-7831-1_17

2018    Gunter, A., Gerencser, A.A., Phuong, L., Bornstein, S., Mookerjee, S.A., Maridas, D., Brand, M.D., Rosen, C. Metabolic Programming of Mesenchymal Progenitor Cells Characterize Lineage Allocation. Journal of Bone and Mineral Research 33(6):1052-1065. doi: 10.1002/jbmr.3390

2017    Gerencser, A.A*, Mookerjee, S.A*., Jastroch, M., Brand, M.D. Positive Feedback Amplifies the Response of Mitochondrial Membrane Potential to Glucose Concentration in Clonal Pancreatic Beta Cells. Biochimica et Biophysica Acta 1863(5): 1054-1065 (doi: 10.1016/j.bbadis.2016.10.015. published online 2016 Oct 20)
    *These authors contributed equally

2017    Mookerjee, S.A., Gerencser, A.A., Nicholls, D.G., Brand, M.D. Quantifying intracellular rates of glycolytic and oxidative ATP production and consumption using extracellular flux measurements. Journal of Biological Chemistry 292(17): 7189-7207

2017    Nguyen, L., Lucke-Wold, B.P., Mookerjee, S.A., Kaushal, N., Matsumoto, R.R. Sigma-1 receptors and neurodegenerative diseases: Towards a hypothesis of sigma-1 receptors as amplifiers of neurodegeneration and neuroprotection. Advances in Experimental Medicine and Biology 964:133-152. doi: 10.1007/978-3-319-50174-1_10.

2016    Gerencser, A.A, Mookerjee, S.A.,  Jastroch, M., Brand, M.D. Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells, PLoS ONE 11(7): e0159199.

2016    Mookerjee, S.A., Nicholls, D.G., Brand, M.D. Determining maximum glycolytic capacity using extracellular flux measurements. PLoS ONE 11(3): e0152016. doi:10.1371/journal.pone.0152016

2015    Guyenet, S., Mookerjee, S., Lin, A., Custer, S.K., Chen, S.F., Sopher, B.L., LaSpada, A.R., and Ellerby, L.M. Proteolytic Cleavage of Ataxin-7 Promotes SCA7 Retinal Degeneration and Neurological Dysfunction. Human Molecular Genetics 2015, 24:3908-3917 (doi: 10.1093/hmg/ddv121. Published online April 2015)

2015    Mookerjee, S.A., Brand, M.D. Measurement and analysis of extracellular acid production to determine glycolytic rate. In press, Journal of Visualized Experiments (106), e53464, doi:10.3791/53464

2014    Nguyen, L., Lucke-Wold, B.P., Mookerjee, S.A., Cavendish, J.Z., Robson, M.J., Scadinaro, A.L., Matsumoto, R.R. Role of sigma-1 receptors in neurodegenerative disease. Journal of Pharmacological Sciences 2015, 127:17-29 (doi:10.1016/j.jphs.2014.12.005; published online December 2014)

2014    Mookerjee, S.A., Goncalves, R.L., Gerencser, A.G., Nicholls, D.G., Brand. M.D. The contributions of respiration and glycolysis to extracellular acid production. Biochim Biophysica Acta 2015, 1847:171-181 (Published online October 2014, http://dx.doi.org/10.1016/j.bbabio.2014.10.005)

2013    Mookerjee, S.A., Sia, E.A. Using genetic reporters to assess stability and mutation of the yeast mitochondrial genome. Directed Mutagenesis 2013 ISBN 979-953-307-450-3 InTech Publishing

2011    Mookerjee, S.A., Brand, M.D. Characteristics of the turnover of uncoupling protein 3 by the ubiquitin proteasome system in isolated mitochondria. Biochim Biophys Acta. 2011. 1807: 1474-81

2010    Brand, M.D., Parker, N., Affourtit, C., Mookerjee, S.A., Azzu, V. Mitochondrial uncoupling protein-2 in pancreatic beta cells. Diabetes Obes Metab. 2010: Suppl 2:134-40

2010    Mookerjee, S.A., Divakaruni, A., Jastroch, M., Brand, M.D. Mitochondrial uncoupling and lifespan. Mech Ageing Dev. 2010. 131: 463-72

2010    Jastroch, M., Divakaruni, A., Mookerjee, S., Treberg, J., Brand, M.D. Mitochondrial Proton and Electron Leaks. Essays Biochem. 2010. 47: 53-67

2010    Azzu, V*., Mookerjee, S.A*., Brand, M.D. Rapid Turnover of Mitochondrial Uncoupling Protein 3. Biochem J. 2010. 426: 13-7 
    *These authors contributed equally

2009    Mookerjee, S., Papanikolaou, T., Guyenet, S., Sampath, V., Lin, A., Vitelli, C., DeGiacomo, F., Sopher, B., Chen, S., La Spada, A., Ellerby, L. Post-translational modification of ataxin-7 at lysine-257 prevents autophagy-mediated turnover of an N-terminal caspase-7 cleavage fragment" J Neurosci. 2009 29:15134-44

2009    Pogorzala, L., Mookerjee, S.A., Sia, E.A. A role for Msh1p in mitochondrial base excision repair Genetics 2009. 182: 699-709 

2006    Mookerjee, S.A., Sia, E.A. Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae. Mutation Research 2006. 595: 91-106 

2005    Mookerjee, S.A., Lyon, H., Sia, E.A. Analysis of the functional domains of the mismatch repair homolog, Msh1p and its role in mitochondrial genome maintenance. Current Genetics 2005. 47: 84-99.

Memberships and Affiliations

Adjunct Associate Professor, Buck Institute for Research on Aging

Teaching Responsibilities

PRMC 601: Biological Sciences I, PRMC 602: Pharmaceutical Sciences II, PRMC 606: Pharmaceutical Sciences II, PRMC 609: Biological Sciences III, PRMC 610: Pharmaceutical Sciences III, PRMC 614: Pharmaceutical Sciences IV, PRMC 636: Science-Based Reproductive Health Care